怀槐树皮异黄酮苷类化学成分研究

Isoflavone glycosides from the bark of Maackia amurensis

研究怀槐(Maackia amurensis)树皮中的化学成分。利用大孔吸附树脂、硅胶、凝胶和ODS柱色谱等分离技术分离化合物,根据化合物的理化性质和光谱数据鉴定其结构。从其70%乙醇提取物的正丁醇萃取部分分离得到了13个异黄酮苷类化合物,分别为4′,6-二甲氧基-异黄酮-7-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷(1),樱黄素-4′-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷(2),芒柄花苷(3),黄豆素苷(4),染料木苷(5),saikoisoflavonoside A(6),阿夫罗摩辛-7-O-β-D-吡喃葡糖苷(7),阿夫罗摩辛-7-O-β-D-木糖-(1→6)-β-D-吡喃葡糖苷(8),4′-甲氧基-异黄酮-7-O-β-D-木糖-(1→6)-β-D-吡喃葡糖苷(9),4′,6-二甲氧基-异黄酮-7-O-β-D-芹糖-(1→6)-β-D-吡喃葡糖苷(10),鸢尾种苷(11),5-羟基-4′-甲氧基-异黄酮-7-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷(12)和4′-甲氧基-异黄酮-7-O-β-D-芹糖-(1→6)-β-D-吡喃葡糖苷(13)。化合物1为新化合物,命名为怀槐异黄酮苷(maackiaisoflavonoside),化合物2,8,9,10,12和13为该属内首次分离得到。

To study the chemical constituents of the Maackia amurensis, the constituents were isolated by various chromatographies and the structures were elucidated on the basis of chemical and spectroscopic data （ESI-MS, 1D and 2D NMR）. Thirteen isoflavone glycosides were isolated from the n-BuOH-soluble fraction of the 70% ethanol extract and identified as 7-hydroxy-4′,6-dimethoxyisoflavone-7-O-β-D-glucopyranosyl-（1→6）-β-D- glucopyranoside （1）, dalsympathetin （2）, ononin （3）, glycitin （4）, genestin （5）, saikoisoflavonoside A （6）, afrorrnosin-7-O-β-D-glucopyranoside （7）, gehuain （8）, kushenol O （9）, 7-hydroxy-4′,6-dimethoxyisoflavone-7-O- β-D-apiofuranosyl-（1→6）-β-D-glucopyranoside （10）, tectoridin （11）, biochanin A 7-O-β-D-gentiobioside （12） and 7- 7-hydroxy-4′-methoxyisoflavone-7-O-β-D-apiofuranosyl-（1→6）-β-D-glucopyranoside （13）. Compound 1 is a new isoflavone glycoside, named as maackiaisoflavonoside, compounds 2, 8, 9, 10, 12 and 13 were isolated from this genus for the first time.