温针灸对大鼠腰神经根受压模型炎性介质NOS和CGRP调节作用的研究

Effects of warm needle moxibustion on nerve root local inflammatory factors (NOS and CGRP) in the lumbar nerve root compress model rats

目的:探讨温针灸对腰神经根压迫损伤的治疗作用机制。方法:采用健康SD大鼠50只,通过放置微型硅胶片方法建立大鼠腰神经根压迫模型,随机分为正常组、模型组(生理盐水治疗)、药物组(莫比可治疗)、针刺组(针刺L5、L6夹脊穴)和温针组(针刺加艾灸L5、L6夹脊穴),治疗14d后切取受压神经根。运用透射电镜观察受损神经根超微结构改变,采用ELISA法检测受损神经根一氧化氮合酶(NOS)、降钙素相关基因肽(CGRP)表达变化。结果:电镜显示,模型组神经根超微结构改变最明显,依次减轻为药物组、针刺组、温针组;温针组压迫区神经根组织内NOS、CGRP的含量较模型组明显减低(P<0.05),与正常组相比差异无统计学意义(P>0.05)。结论:温针治疗可以有效维持神经根背根节细胞形态、超微结构,有效抑制炎症介质NOS、CGRP的释放。

Objective To explore the mechanism of acupuncture for treatment of lumbar nerve root compression injury. Methods Fifty healthy SD rats were randomly divided into 5 groups, a normal group, a model group treated by saline, a medication group treated with Caerulein, an acupuncture group treated with acupuncture at L5 ,L6 Jiaji （EX-B 2） and a warm needle group treated with acupuncture and moxibustion at L5 ,L6 Jiaji （EX-B 2）. The lumbar nerve root compress injury model was made by placing microsilica gel tablet. After they were treated for 14 days, the compressed nerve root was taken and the ultra microstructure changes of the injured nerve root were observed by electron microscope and changes of nitric oxide synthase （NOS） and calcitonin gene-related peptide （CGRP） expressions were investigated by ELISA assay. Results The changes of ultra-microstructure of the nerve root were the most obvious in the model group and the changes in the medication group, the acupuncture group and the warming needle group reduced in order; the NOS activity and CGRP content in the nerve root tissue of the compressed area in the warm needle group were significantly reduced as compared with the model group （P〈0.05）, but with no significant difference as compared with those in the normal group （P〉0.05）. Conclusion Warm needle treatment can effectively maintain cellular form, and uhra-microstructures of nerve root dorsal root ganglia, and effectively inhibit the release of inflammatory factors NOS and CGRP.