摘要
目的用高效液相色谱法鉴别五味子与南五味子。方法采用RP-HPLC,以五味子甲素峰为参考峰,色谱柱:DIKMADIAMONSIL C18柱(4.6 mm×150 mm,5μm);流动相:甲醇-水(65∶35);检测波长:254 nm。结果在选定的色谱条件下,对五味子与南五味子进行系统聚类与判别分析,提供了稳定可控的液相色谱图。结论能够满足鉴别五味子与南五味子的要求;采用系统聚类与判别分析和液相色谱技术鉴别五味子与南五味子是切实可行的。
OBJECTIVE To identify fuctus schisandrae chinensis and fructus schisandrae sphenantheae by RP-HPLC. METHODS DIKMA DIAMONSIL C ms column（4.6mm×150mm,5μm）was used. The mobile phase was metharrol-water（65：35 ） and the detection wavelength was 254 nm. RESULTS Using above optimum chromatography conditions, the HPLC of relative medicinal materials was established. CONCLUSION HPLC can be used for identification of fruetus schisandrae chinensis and fructus schisandrae sphenantherae by Cluster Analysis and Discriminant Analysis.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第1期29-35,共7页
Chinese Journal of Modern Applied Pharmacy
关键词
高效液相色谱法
五味子
南五味子
系统聚类分析
判别分析
HPLC
fruetus schisandrae ehinensis
fruetus sehisandrae sphenantherae
cluster analysis
discriminant analysis
