正交设计优化八氟丙烷白蛋白微球混悬液的制备工艺

Optimizing Preparation of Encapsulated Octafluoropropane Albumin Microspheres by Orthogonal Design

目的优化八氟丙烷白蛋白微球混悬液的制备工艺。方法以微球的平均直径和浓度为考察指标,以八氟丙烷通气量、声振强度和终点温度作为工艺条件进行三因素三水平的正交试验。结果最佳工艺条件为采用1%人血白蛋白溶液20mL,声振强度90%,5 s内通入八氟丙烷2 mL,终点温度77℃。结论采用该最佳工艺制备的八氟丙烷白蛋白微球混悬液,微球浓度高而且稳定。

OBJECTIVE To optimize preparation of encapsulated oetafluoropropane albumin mierospheres. METHODS The microspheres in suspension were produced by sonication of 20 mL of 1% human serum albumin solution in the presence of octafluoroprane gas. Orthogonal design was used for studying the octafluoropropane gas flow rate, sonication intensity and process temperature of termination on the diameter and concentration of microspheres. RESULTS The best optimization process was obtained as follows： 90 % intensity as the sonieation power, 2 mL per 5 s as the gas flow rate,77 ℃ as the terminal process temperature. CONCLUSION The microspheres prepared by this method are stable.