不稳定性心绞痛血瘀证的血浆蛋白质组学研究

Plasma Proteomics Study on Unstable Angina Patients with Blood Stasis Syndrome

为了寻找冠心病不稳定性心绞痛血瘀证血浆差异表达蛋白,探索冠心病不稳定性心绞痛血瘀证的蛋白质组学特点,采用差异凝胶双向电泳和质谱联用技术对12例冠心病不稳定性心绞痛血瘀证患者和12例健康人血浆进行比较研究.初步发现了Fibrinogen β chain,Fibrinogen γ chain,α1-Antitrypsin,Haptoglobin β chain,Haptoglobin α2 chain在冠心病不稳定性心绞痛血瘀证患者中高表达,ApoA-IV,ApoA-I,Transthyretin,ApoJ在冠心病不稳定性心绞痛血瘀证患者中低表达.差异表达蛋白根据功能可分为以下三类:(1)急性时相反应负相蛋白;(2)载脂蛋白;(3)凝血相关蛋白.冠心病不稳定性心绞痛血瘀证可能与炎症反应、脂代谢紊乱以及凝血功能异常相关.

To seek differentially expressed plasma proteins of unstable angina patients with blood stasis syndrome, two-dimensional difference gel electrophoresis and mass spectrometry were used to screen the differentially expressed plasma proteins among such patients with blood stasis syndrome and healthy volunteers. Fibrinogen β chain, fibrinogen y chain, a1-antitrypsin, haptoglobin β chain, haptoglobin a2 chain were significantly highly expressed in the plasma of these patients, while ApoA-IV, ApoA-I, transthyretin and ApoJ were decreased in their plasma. These identified increased expressed proteins could be divided into three categories according to their functions： （1） acute phase reactive protein, （2） apolipoprotein, （3） blood coagulation protein. Unstable angina with blood stasis syndrome may be correlated with inflammatory reaction, lipid metabolic disorder and blood coagulation factor abnormality, and these differentially expressed proteins could provide clues for the study of unstable angina biomarkers and discovery of new protein targets for antianginal drugs.