正交试验法优选田七花总黄酮的提取工艺

Optimization of Extraction Process of Total Flavonoids of Flowers of Panapx notoginseng with Orthogonal Experiment

用正交试验法对田七花中的总黄酮提取工艺进行优化.以田七花中的总黄酮含量为考察指标,采用正交试验L9(34)表进行实验,对提取过程中的乙醇浓度、提取时间、提取次数及料液比四个影响因素进行研究.结果显示影响田七花中的总黄酮提取工艺的显著因素是提取次数,最佳提取工艺是:12倍量的80%的乙醇提取3次,每次1h.

In order to optimize the extraction process of the total flovonoids from the flowers of Panapx notoginseng, fhe extraction process of total flavonoids was studied by orthogonal experiment. The orthogonal experiment was employed to check influences of four factors：concentration of alcohol, extraction time, frequency of extraction and ratio of solvent to material, selecting amount of flavonoids as a marker according to L9（3^4）scheme. The frequency of extraction and extraction time have a significantly impact on the extraction of total flovonoids from the flowers of Panap.r notoginseng. The optimum extraction process was A3B2C1D3 ： the extraction was run three times each hour,and the aqueous solution of 80% alcohol was used as an extraction solvent.