血清HBsAg阳性患者肝癌及癌旁组织中HBx基因的表达及p53基因的突变

Expression of HBx gene and mutation of p53 gene in HCC tissues of serum HBsAg+ patients.

本实验用ＰＣＲ、ＰＣＲ－ＳＳＣＰ银染方法对２４例血清ＨＢｓＡｇ（＋）患者肝癌及癌旁组织中ＨＢｘ基因表达和ｐ５３基因突变进行了检测。肝癌组织中ＨＢｘ基因表达率为１７％（４／２４），ｐ５３基因突变率为３３％（８／２４），癌旁正常组织和硬化组织中均无ＨＢｘ基因表达和ｐ５３基因突变。存在ＨＢｘ表达的肝癌组织中ｐ５３基因突变率为１００％（４／４），ｐ５３基因突变与ＨＢｘ基因表达具有相关性（Ｐ＜０．０１）。

PCR and PCR-SSCP were used to study p53 gene mutation and HBx gene expression in HCC tissues and tumor-adjacent tissues of serum HBsAg(+) with.The rate of p53 gene mutation was 33% in HCC tissues(8/24),the rate of HBx gene expression was 17%(4/24).Neither p53 mutaton nor HBx gene was founded in tumor-adjacent tissues.The rate of p53 gene mutation was 100%(4/4) in tumor tissues with HBx gene.p53 gene mutation was congratulated with HBx gene expression.It was suggested that the mechanism of p53 mutation was associated with intergrated HBx gene.