白细胞介素2镇痛功能位点氨基酸残基相对空间位置的重要性研究

IMPORTANCE OF TERTIARY LOCATION OF THE AMINORESIDUES RESPONSIBLE FOR ANALGESIC DOMAIN OF INTERLEUKIN2

免疫调节因子白细胞介素－２（ＩＬ－２）具有中枢镇痛作用，其功能位点由ＩＬ－２分子的第４４、４５和１０７、１１７位氨基酸残基共同组成。采用基因定点突变和ＰＣＲ技术，缺失ＩＬ－２分子第６６～９９位残基，改变第４４、４５位残基与１０７、１１７位残基的相对空间位置，发现其镇痛功能消失。结果提示了组成ＩＬ－２分子镇痛功能位点的氨基酸残基间相对空间位置排列的重要性。

Interleukin2(IL2), an immune regulator, is also an analgesic molecule. The function domain is composed of the aminoresidues of 44th, 45th and 107th, 117th and so on in IL2 molecule. Using sitedirected mutagenesis and PCR methods, aminoresidues from the 60 th to 99th in IL2 molecule were deleted(Del60～99IL2) to alter the relative tertiary location. The analgesic activity of Del60～99IL2 was disappeared. The results suggested that the relative tertiary location is important to the analgesic function among the residues responsible for analgesic domain of IL2.