荷癌大鼠血中高凝状态和早期血栓形成的免疫组织化学研究

A IMMUNOHISTOCHEMICAL STUDY ON THE HYPERCOAGULABLE STATE AND THE EARLY THROMBOSIS IN TUMOR BEARING RAT

通过血液学检查及应用羊抗大鼠纤维蛋白原抗体的荧光免疫组织化学方法，对实验组动物凝血系统改变及在组织中对纤维蛋白原抗原的表达进行探讨。结果发现：实验组动物血液中血小板数减少、纤维蛋白原浓度增高。荧光免疫组织化学观察可见在肺、肾、心、脑、肝及肿瘤组织血管内呈阳性反应。证明免疫荧光组织化学检查可明确显示血中高凝状态及早期血栓形成，这对于判断早期血栓形成及分析血栓变迁过程，具有重要临床意义。

To determine the morphological evidence of hypercoagulable state and early thrombosis, the examinations of hematological tests and immunohistochemistry with antifibrinigen antibody were performed in a newlydeveloped chemotherapy treated tumor bearing rats.The decreasing of platelet count and the increasing of fibrinogen level were shown in experimental group.The accumulation of fibrinogen was determined immunohistochemicaly and histochemicaly where from it is leading to early thrombosis.