CD4^+CD45^+T细胞对SLE病人B细胞产生自身抗体的调节作用

REGULATION OF CD4+ CD45+ T CELLS ON THE GENERATION OF AUTOIMMUNE ANTIBODIES OF B CELLS IN SLE PATIENTS

选择体外抗ＤＮＡ、抗ＣＤＬ抗体双阳性的ＳＬＥ病人Ｂ细胞做为靶细胞，分别与不同的ＳＬＥ病人或正常人ＣＤ４＋ＣＤ４５＋Ｔ细胞亚群共同培养。结果显示，ＳＬＥ病人ＣＤ４＋ＣＤ４５ＲＯ＋Ｔ细胞对自身抗体的产生有明显的辅助作用，而ＣＤ４＋ＣＤ４５ＲＡ＋Ｔ细胞的上述作用不明显。健康人ＣＤ４＋ＣＤ４５ＲＯ＋Ｔ细胞及ＣＤ４＋ＣＤ４５ＲＡ＋Ｔ细胞对自身抗体的产生均有抑制作用。还观察到，病人Ｂ细胞在接受ＣＤ４＋ＣＤ４５ＲＯ＋Ｔ细胞的调控实验中，必须利用合适的ＤＮＡ、ＣＤＬ抗原浓度。

B cells of SLE patients which could secret antiDNA and antiCDL antibodies in vitro were selected as target cells.These target cells were respectively cultured with different T cell subsets of CD4+,CD4+ CD45+ of SLE patients or of normal persons.The results demonstrated that CD4+ CD45+ T cells had the obviously auxiliary effect on the generation of autoimmune antibodies,but the abovementioned effect of CD4+ CD45RA+ T cells was not obvious.CD4+ CD45RO+ T cells and CD4+ CD45RA+ T cells all had inhibitory effect on the generation of autoimmune antibodies in normal persons.It had also been observed that B cells of SLE patients were regulated and controlled by CD4+ CD45RO+ T cells,and at the meantime that these cells were effected by antigens such as DNA,CDL and so on.