抗卵细胞核抗体的研究

ANTIBODY TO NUCLEAR OF EGG CELL

系统性自身免疫病患者体内出现的抗核抗体（ＡＮＡ）可与多种体细胞的胞核发生反应已有较多研究，但其是否可以与卵细胞核发生反应的研究尚少见报道。以鸡卵核为抗原建立了酶联免疫法，检验相应抗体。结果表明：体细胞ＡＮＡ阳性组卵核ＡＮＡ的阳性率为８３．３％（１５／１８），体细胞ＡＮＡ阴性组卵核ＡＮＡ的阳性率为２３．３％（７／３０），两者具有显著差异（Ｐ＜０．０１），从而证实ＡＮＡ亦可与卵细胞胞核发生反应。同时对文中建立的检测卵核ＡＮＡ方法的主要质控指标进行研究测试，表明该法稳定可靠。对其理论意义及应用价值进行讨论。

Antinuclear antibody, ANA, was able to react with varriable somatic cells. There was not report about ANA reacting with nuclear of egg. Antibody to nuclear of egg was detected by ELISA coated with yolk. The result showed that positive rate was 83.3%(15/18) in positive group of ANA to liver cell in white rat. It was 23.3%(7/30) in negative group. There was significante difference between two groups(P<001). It was suggested that ANA was able to react with nuclear of egg. The significations and practical value were discussed.