肿瘤的转移潜能与nm23/NDPK活性、蛋白含量及mRNA表达的相关性研究

Relationship of Metastatic Potevtial with ActiuityContent and mRNA Level of nm23/NDPK

对本研究室自建的小鼠前胃癌（ＭＦＣ）细胞系、株和小鼠树突状细胞肉瘤（ＤＣＳ）细胞系、株的移植瘤组织和培养细胞进行ｎｍ２３／ＮＤＰＫ活性、免疫组化染色及ＲＮＡ杂交的研究。－３２ＰＵＴＰ生成法和乳酸脱氢酶一丙酮酸激酶偶联法均证明这些瘤组织或瘤细胞存在ＮＤＰＫ活性。ＭＦＣ、株转移率高者ＮＤＰＫ活性低，反之则高；而ＤＣＳ系、株瘤组织ＮＤＰＫ活性高、低与瘤株转移率高、低无明显规律性关系。ＭＦＣ和ＤＣＳ各株细胞的ＮＤＰＫ免疫组化染色呈弱阳性，ｎｍ２３ＲＮＡ狭缝杂交（地高辛标记探针）均呈阴性。结果提示ｎｍ２３／ＮＤＰＫ对ＭＦＣ可能具有转移抑制的功能。

The nm23/NDPK was studied for its activity, content and mRNA level in both mouse forestomach carcinoma cell line (MFC)and mouse dendritic cell sarcoma cell line (DCS). The NDPK activity was consistently identified in these tumor tissues or cells using assay of r32P UTP product and coupled pyruvate lactate dehydrogenase system. It was found that NDPK activity demonstrated an inverse relationship with the metastatic potential of the substrains from MFC, but variably related with that from the DCS. The cells of both MFC and DCS had weak positive staining immunohistochemically and negative in RNA slot hybridization using DIGlabelled probe. The results indicate that nm23/NDPK might play an important inhibitive role in the metastatic potential of MFC, but not in DCS. 