家兔早期酒精性股骨头坏死的生物化学改变

Biochemical changes in the early stage of alcohol-induced avascular necrosis of the femoral head of rabbits

为探讨长期过量饮酒时家兔血清生物化学改变及其在股骨头缺血性坏死（ANFH）中所起的作用。取健康家兔80只，随机分为实验组和对照组，每组40只。采用灌胃法，实验组给予烈性酒（（乙醇）＝45％）10ml／（kg·d），对照组给予100g／L的葡萄糖溶液10ml／（kg·d）。2组动物在实验第1、2、3、6个月时分批处死。结果显示：实验组动物血清LPO、GGT、ALT、AST、TG和CH升高，SOD降低；病理组织学检查见脂肪肝和股骨头缺血性坏死呈早期改变。提示：生化代射异常变化与酒精性ANFH的发生发展密切相关。

To study the biochemical changes and the pathogenesis of avascular necrosis of the femoral head (ANFH) after long-term excessive intake of spirits, 80 New Zealand rabbits were used. The animals were divided at random into the experimental group and the control group of 40 animals each. By pouring into stomach, the experimental group was administered 10 ml/(kg·d) spirits, which contains 45% ethanol,and the control group was given the same dose of 10% glucose solution. The animals were sacrificed in batches at the 1st, 2nd, 3rd and 6th month after treatment. The results showed that in the experimental group, the levels of serum lipid peroxides (LPO), gamma glutamyl transferase (GGT), aspartate amino transferase(AST), alanine amino transferase(ALT), triglycerid(TG) and cholesterol (CH) increased, and the level of superoxide dismutase(SOD) reduced. There was a significant difference between the experimental and the control groups (P < 0. 01 ). The changes of histology, fatty liver and the changes in the early stage of ANFH were seen after the treatment. All of these changes were not seen in the control group. The results suggest that the abnormal changes of the biochamical metabolism may have a close relation to the development of alcohol-induced ANFH.