摘要
目的:探讨钙耐受大鼠心肌细胞的快速、高效分离方法。方法:采用自行改进的Langendorff心脏灌流装置,逆行主动脉恒流灌注获得单个耐钙心肌细胞,KB液温育后以全细胞膜片钳方式记录离子流。结果:分离所得70%存活心肌细胞有80%为钙耐受心肌细胞,可记录到典型的瞬时外向钾电流。结论:根据灌注压力变化掌握消化时间,控制好细胞分离过程的各种因素,能够获得大量具有正常电生理特性的钙耐受心肌细胞。
OBJECTIVE： To investigate the method for rapid and effective isolating of calcium - tolerant cardiomyocytes （CTC） from rats. METHODS： CTC were obtained by retrograde perfusion using modified Langendorff heart perfusion apparatus. After incubation of cardiomyocytes in KB solution, the ion currents were recorded with patch - clamp technique in whole - cell mode. RESULTS： Of the whole isolated cells, 70% were survival. Of which, 80% were CTC. A typical outward potassium current was recorded. CONCLUSIONS： A large number of CTC with normal electrophysiological characteristics can be obtained by mastering the digestion time based on variation of perfusion pressure and keeping all the factors in cell isolation under control.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第4期254-255,共2页
China Pharmacy
关键词
大鼠
钙耐受
心肌细胞
分离
膜片钳
Rat
Calcium tolerant
Myocardial cell
Isolation
Patch clamp
