云南4个少数民族带绦虫分子鉴定研究

Research on molecular identification of taenia cestode from 4 ethnic populations in Yunnan Province

目的利用分子遗传标志对采自云南西部彝族（楚雄）、傈僳族（怒江）、普米族（怒江）、白族（大理）等4个少数民族带绦虫标本作生物多态性的研究。方法取云南彝族分离株、傈僳分离株、普米分离株、白族分离株和贵州从江分离株带绦虫成虫节片，提取线粒体DNA，PCR扩增线粒体DNA细胞色素B（mtDNA—Cytb）序列部分片段测序；结合GenBank中已知猪带绦虫、牛带绦虫和牛带绦虫亚洲亚种mtDNA—Cytb序列，使用PHYLIP软件包运用最大似然法和最大简约法构建系统发育树。结果系统发育树显示云南傈僳族、普米族和白族带绦虫标本与台湾牛带绦虫亚洲亚种的遗传距离最接近，距云南彝族、贵州从江牛带绦虫标本较远，与猪带绦虫则更远。结论云南傈僳族（怒江）、普米族（怒江）和白族（大理）带绦虫标本属于牛带绦虫亚洲亚种，云南彝族（楚雄）带绦虫标本属于牛带绦虫；mtDNA—Cytb序列分析可以用于带绦虫生物多态性研究。

Objective To study the biologic polymorphism of taenia cestode samples from Yi population （Chuxiong）, Lisu population （Nujiang）, Pumi population （Nujiang） and Bai population （Dali） in the west of Yunnan by molecular genetic markers. Methods Mitochondrial DNA were extracted from the proglottis of adult taenia cestode strains from Yi population, Lisu population, Pumi population, Bai population and Congjiang District respectively, partial segments of mitochondrial DNA-cytochrome B（mtDNA-Cytb） sequences were amplified by polymerase chain reaction （PCR）, then sequenced. Phylogenetic tree of species and geographical isolates was constructed with other known mtDNA-Cytb sequence of T. solium, T. saginata and T. saginata asiatica in GenBank. Results The phylogenetic tree constructed revealed that the taenia cestode samples from Lisu population, Pumi population and Bai population in Yunnan Province were close to Taiwan strain genetically, but far from Yi population in Yunnan Province and Congjiang District in Guizhou Province, even farther from T. solium. Conclusion The taenia cestode samples from Lisu population （ Nujiang）, Pumi population （Nujiang） and Bai population （Dali） in Yunnan Province belong to T. saginata asiatica, the taenia cestode sample from Yi population （Chuxiong） in Yunnan Province belongs to T. saginata, mtDNA-Cytb sequence analysis could be used in the study on biologic polymorphism of taenia cestode.