蒲公英花黄色素的超声提取工艺及叶黄素含量测定

Ultrasonic extraction of yellow pigment from Dandelion′s petal and quantitative determination of its lutein

从野生蒲公英花中提取的黄色素是一种安全、无毒的天然色素,因其中含有一定量的叶黄素,故其具有很高的营养价值。利用正交设计研究了该色素的超声提取工艺,并且对提取的黄色素用HPLC(高效液相)法进行了叶黄素含量测定。提取最佳条件为提取时间90min、提取温度55℃、提取功率60W;HPLC条件:使用十八烷键合硅胶色谱柱,流动相为乙腈∶甲醇(95∶5),检测波长为446nm。该方法在3.6～18.0μg/mL范围内线性关系良好,加样回收率为100.03%。

The yellow pigment extracted from Dandelion＇s petal is safe and nonpoisonous. To consist of a lot of lutein it is also a nature pigment with certain nutritive value. We studied ultrasonic extraction and quantitative determination of lutein by HPLC. The parameters of optimum extraction process： frequency of ultrasound to extracting is 60 W, extractible temperature is 55 ℃, extractible time is 90 minutes. The HPLC used Diamonsil C18 column, a mixture of （CH3CN：CH3OH=95：5） as mobile phase, and UV detection at 446 nm. Lineal relation is better when the sample is 3.6-18.0 μg/mL. The recovery of added lutein is 100.03%.