华美二号猕猴桃根提取物保肝作用的实验研究

Experimental research on ethyl acetate extract of kiwifruit root in injured mice liver protection

目的:了解观察华美2号猕猴桃根乙酸乙酯提取物部分(简称ERHM部位,主要是三萜类化合物)的保肝作用。方法:釆用ERHM对四氯化碳(CCl4)、D-半乳糖胺和异硫氰酸萘酯(ANIT)致小鼠急性肝损伤保护实验、单核巨噬细胞吞噬功能实验、免疫低下小鼠溶血素生成实验和毒性实验;血清中的ALT、AST、T-BIL含量作为测定指标。结果:与CCl4、D-半乳糖胺和ANIT模型组相比,ERHM(100mg/kg,50mg/kg)显著降低ALT、AST、T-BIL含量(P<0.05)。单核巨噬细胞吞噬功能实验表明,ERHM(100mg/kg)显著提高单核巨噬细胞吞噬指数(P<0.05)。溶血素生成实验表明,ERHM(100mg/kg,50mg/kg)显著提高免疫低下小鼠溶血素含量(P<0.05)。限量毒性实验表明,安全无毒。结论:ERHM具有较好的保肝降酶和退黄、提高免疫功能作用,安全无毒。

To study effect of ethyl acetate extract of kiwifrui root（ERHM）on liver protection. The experimental mouse models induced by carbon tetrachloride（CCl4）, D-galactosamine（D-Gla）and naphthyl isothiocyanate （ANIT）are used. The assay of mononuclear macrophage phagocytosis and hemolysin formation in immunelowering mice and toxicity test are also conducted, ALT, AST and T-BIL are tested as indicators. Compared with CCl4, D-Gla and ANIT models, ERHM at 100 mg/kg and 50 mg/kg can decrease the contents of ALT, AST and T-BIL at P〈0.05. ERHM at 100 mg/kg can increase phagocytosis index of mononuclear macrophages at P〈0.05. ERHM at 100 mg/kg and 50 mg/kg can increase the content of hemolysin in immune -lowering mice at P〈0.05. It is suggested that ERHM could enhance immunity and protection of liver, and eliminate jaundice.