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富勒烯膦酸衍生物纳米水悬液对DNA限制性内切酶的抑制作用及其机理 被引量：4

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摘要 富勒烯纳米颗粒水悬液的生物学效应正在引起人们的极大关注.采用具有EcoRⅠ、BamHⅠ、Cfr9Ⅰ和XmaⅠ单一酶切位点的pEGFP-N1超螺旋型质粒为底物,检测了单加成亚甲基富勒烯[60]二膦酸四乙酯（mono-methanophosphonate fullerene,MMPF）和二加成亚甲基富勒烯[60]二膦酸四乙酯（bis-methanophosphonate fullerene,BMPF）的纳米水悬液（分别表示为n-MMPF和n-BMPF）对各种DNA限制性内切酶的抑制作用.实验发现,n-MMPF和n-BMPF均对EcoRⅠ有抑制作用,但后者的作用更强些.加入n-BMPF后,对BamHⅠ酶切反应的半抑制浓度IC50值大于30μmol/L,而对EcoRⅠ的半抑制浓度IC50值仅为4.3μmol/L,对同工酶Cfr9Ⅰ和XmaⅠ的半抑制浓度IC50值分别为11.7和8.3μmol/L.当EcoRⅠ酶切反应完全被n-BMPF抑制时,在反应体系中增加底物pEGFP-N1的量,并不能观察到酶切产物线型质粒,但是在酶切体系中增加酶的量则能拮抗n-BMPF的作用.两种活性氧清除剂甘露醇和叠氮化钠在浓度为2-10mmol/L时,不能拮抗n-BMPF的作用,说明n-BMPF的抑制活性与活性氧无关.这些结果首次表明,富勒烯纳米颗粒水悬液具有作为DNA限制性内切酶抑制剂的生物活性.
作者 宋高广 姚璐 黄成 解馨 谭信 杨新林
机构地区 北京理工大学生命科学与技术学院
出处 《中国科学（B辑）》 CSCD 北大核心 2009年第1期61-67,共7页 Science in China(Series B)
基金 国家自然科学基金(批准号:20672012) 北京市自然科学基金(批准号:2082020)资助
关键词 二膦酸四乙酯 纳米颗粒水悬液 DNA 限制性内切酶 活性氧
分类号 Q55 [生物学—生物化学]
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参考文献25

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二级参考文献39

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中国科学（B辑）
2009年 第1期

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