海洋沉积物中反硝化细菌的分离鉴定及反硝化性能研究

Identification and Denitrification Analysis of Denitrifying Bacteria in Sea Sediment

利用良好的通气条件对反硝化细菌进行富集筛选,同时利用二苯胺试剂和Griess试剂显色反应分别检测NO3--N的降解和NO2--N的产生情况,并结合产气量筛选出具有良好耐氧性且反硝化性能优异的菌株.最终从南海海域沉积物中筛选得到具有较强反硝化能力的细菌,并对目的菌株进行分子鉴定及反硝化能力测试.结果表明,目的菌株F-8-1能较好地降解培养基中的NO3--N和NO2--N,产气快.气相色谱检测发现,气体样品中的φ(N2)非常高,接近100%,且未检测出其他氮氧化物气体.16SrDNA序列分析表明,菌株F-8-1与假单胞菌属具有99%的序列相似性和很高的同源性.菌株F-8-1在有氧及高盐环境中生长良好.

Denitrifying bacteria were enriched and screened in good ventilation conditions. Then, using a color reaction of diphenylamine and Griess reagents, nitrate degradation and nitrite production were detected respectively, and, combining with gas production, denitrifying bacteria with superior performance were selected. Finally, denitrifying bacteria were isolated from sediment of the South China Sea, and denitrification analysis and molecular identification were carried out. The results indicated that F-8-1 degraded nitrate and nitrite much better and produced gas earlier. The results of gas chromatography showed that the gas production of F-8-1 was high purity nitrogen, close to 100%, and there were not other nitrogen-oxygen compound gases detected. This indicated that the strain F-8-1 had strong denitrification capacity. The phylogenic analysis based on 16S rDNA and performed by BioEdit and MEGA showed that the strain F-8-1 was the closest relative of Pseudomonas with 99% sequence similarity and high homology. Furthermore, F-8-1 can grow well in aerobic and high salinity environment; therefore, the strain has good prospects.