纤维素衍生物涂敷型高效液相色谱手性固定相的制备及手性拆分研究

Preparation of Chiral Stationary Phases Coated Using Cellulose Derivatives and Study of Chiral Resolution in HPLC

合成了纤维素－三（苯甲酯）（ＣＴＢ）和纤维素－三（氨基苯甲酸酯）（ＣＴＰＣ）衍生物，并涂敷于自制的球形硅胶上（５μｍ，１３ｎｍ），制备了用于高效液相色谱手性拆分的固定相．考察了涂敷量、流动相等因素对手性拆分的影响，测定了一个不对称氢化酯化反应的对映体过剩值（ｅ．ｅ．值）．

Optical resolution of a range of racemic compounds was investigated using cellulose tribenzate and trisphenylcarbamate coated onto a small partial spherical organosilane modified silica. The influence on chiral discrimination of factors such as coated loadings and mobile phase was performed. The loadings of 15% on the support gave the best results. The regioselectivity of an asymmetric hydroesterification of styrene was determined by HPLC using a chiral stationary phase(CTPC CSP). A satisfactory result had been obtained on CTPC chiral column with n hexane/2 propanol(98/2 in volume ratio) as mobile phase.