β-环糊精构筑氨基酸氧化酶的研究

Studies of Amino Acid Oxidase Constructed by β-Cyclodextrin Derivant

报道了用β-环糊精二间羧基苯磺酸酯与三氯化铁形成的配合物构筑模拟氨基酸氧化酶,将苯丙氨酸氧化脱氨生成苯丙酮酸,在过量H_2O_2的存在下,苯丙酮酸进一步被氧化脱羧生成苯乙酸,若此反应体系是在N,N-二甲基甲酰胺(以下缩写DMF)的弱碱性条件下。

Mimic amino acid oxidase was synthesized by the reaction of β - cyclodextrin, 3 -(chlorosulfonyl) benzoic acid with iron trichloride. The synthesis of phenyl pyruvic acid was accomplished by oxidative deamination of mimic enzyme to phenylalanine in water and tyrosine can be obtained by mimic enzyme to phenylalanine in N, N - dimethylformamide. Phenylacetic acid can be obtained by oxidative decarboxylation of phenyl pyruvic acid catalyzed by mimic enzyme in hydrogen peroxide.