毛细管电泳分离氯代酚的研究

Separation of Chlorophenols by Capillary Zone Electrophoresis

用毛细管区带电泳（ＣＥ）方法进行了氯代酚异构体分离的研究。使用 ６４．５ ｃｍ × ５０μｍｌ．Ｄ．石英毛细管柱，在４０％（Ｖ／Ｖ）有机溶剂（如甲醇，乙腈等）改性的磷酸盐缓冲溶液中，通过调节最佳ｐＨ值，达到了一次进样对所有氯代酚的基线分离。

Separations of chlorophenol isomers have been studied by capillary zone elec- trophoresis. A capillary column with 64. 5 cm in length and with an inner diameter of 50 μm was used. All chlorophenol isomers were baseline separated for less than 16 min by adding organic modifiers(methanol, acetonitrile, etc. ) in phosphate buffer. Organic modifiers and pH values of the applied buffer are two important factors for the separation of all chlorophe- nols. The pH values of the run buffers, the more important factor than that of organic modi- fiers, can be optimized. The optimum value of pH is 7. 8 for all organic modifiers with differ- ent compositions.