中性溶质在反相毛细管电色谱中的保留行为

Retention Behaviours of Neutral Solutes in Reversed Phase Capillary Electrochromatography

分别在以甲醇、乙腈、异丙醇和四氢呋喃为有机改性剂的４种二元流动相体系中对中性溶质在反相毛细管电色谱中的保留行为进行了研究。不仅考察了有机改性剂的种类和浓度对电渗淌度的影响，而且建立了溶质容量因子与有机改性剂在流动相中体积分数间的定量关系，此外还对样品在反相毛细管电色谱和反相毛细管高效液相色谱中的保留行为进行了比较，发现中性溶质在这两种分离模式中的容量因子基本相同。

In this paper, retention behaviours of neutral solutes in reversed phase capillary electro chromatography (RP CEC) was studied separately with methanol, acetonitrile, isopropanol and tetrahydrofuran as organic modifiers. It was found that at certain concentration, the order of μeo was in accordance with the ε/η value of the organic modifier in the mobile phase. In addition, the relationship between μeo and φ was relevant to the property of the organic modifier. With more organic modifier in the mobile phase, μeo increased in methanol water and acetonitrile water systems; it changed little in tetrahydrofuran water system and decreased slightly in isopropanol water system. Linear relationship between capacity factors of neutral solutes and the volume fraction of the organic modifier in the mobile phase was obtained in CEC, and was similar to that in RP HPLC. With the same column under the same conditions, it was found that the capacity factors of solutes in RP CEC and RP HPLC were almost the same, which means that the retention mechanisms of neutral solutes in these two modes might be quite similar.