手性色谱固定相——全甲基化2,3,6-三(O-2′-混旋羟丙基)-β-环糊精的立体选择性研究

Study on Enantioselectivity of Chiral Stationary Phase— Permethyl2,3,6 Tri(O 2′ Racemic Hydroxypropyl) β Cyclodextrin

合成了一种新的手性色谱固定相——全甲基化２，３，６－三（Ｏ－２′－混旋羟丙基）－β－环糊精（ＰＭＲＨＰ－β－ＣＤ），以静态法将其涂于０．２５ｍｍ×１６０００ｍｍ弹性石英毛细管柱上，以醇、氨基酸、酯、卤代烃等手性化合物考察了新固定相对对映异构体的拆分能力。结果表明，新的固定相具有较高的立体选择性。

A highly selective chiral stationary phase (CSP), permethyl 2,3,6 tri( O (S) 2 hydroxypropyl β cyclodextrin (PMHP β CD) synthesized by using R (S ) propylene oxide is reported. In this paper, another CSP, permethyl 2,3,6 tri( O 2 racermic hydroxpropyl) β cyclodextrin (PMRHP β CD) was synthesized by using racemic propylene oxide and characterized by TLC, IR and NMR. The PMRHP β CD was coated on a fused silica capillary column(0.25mm×16m). The enantiomers, including alcohols, amino acid, ester and so on were used to test its enantioselectivity. The experimental results show the high selectivity of this cyclodextrin derivative. It almost can be used as a chiral stationary phase as with PMHP β CD in capillary GC, especially for alcohols and different derivatization methods gave different separation results.