毛细管电泳法测定非水溶性药物——复方降压片

Determination of Hydrophobic Pharmaceutical:Tablet of Reserpine Compound by Capillary Zone Electrophoresis

在区带电泳中，应用在背景电解质中加入乙腈的方法对复方降压片的两种主要成分利血平、双氢氯噻嗪进行了分离和定量测定。５次重复测定的相对标准偏差小于３％，检出限分别为０．２６９ｍｇ／Ｌ和１．８４ｍｇ／Ｌ，并对ｐＨ值、磷酸盐浓度及乙腈用量进行了考察。

Tablet reserpine compound is a kind of hydrophobic pharmaceutical that can not be separated by CZE with saltwater system. We solved this problem by adding organic component into the buffer. Thus a satisfactory separation of reserpine and hydrochlorothiazide was achieved by using a 24% (V/V) acetonitrile in the buffer solution of 10 mmol/L phosphate with a pH 9.0. The capillary column of 50μm i.d. and 50 cm total length with effective length(the distance from the inlet to the detector) of 35cm was used during all analytical procedure. The factors which influenced resolution such as the pH, acetonitrile content and phosphate concentration have been investigated. The CZE separation conditions of reserpine and hydrochlorothiazide using the standard mixture solution. are also showed. In the quantitative analysis, the reproducibility of CZE determination is illustrated. The regression coefficients of calibration graphs and the detection limits are given. The results of the study of recovery and quantitative results of these components using external standard method are given.