反相高效液相色谱法测定杜仲中的松脂醇二葡萄糖甙

A ReversedPhase High Performance Liquid Chromatographic Method for the Determination of Pinoresinol Diglucopyranoside in Eucommia Ulmoides Oliv

建立一种测定杜仲中松脂醇二葡萄糖甙的反相高效液相色谱法。杜仲粉末的甲醇提取液经大孔树脂柱处理后，在ＹＷＧ－Ｃ１８柱上进行ＨＰＬＣ分析。流动相为２８％（Ｖ／Ｖ）的甲醇水溶液；流速１．０ｍＬ／ｍｉｎ；紫外检测波长２３２ｎｍ。方法平均回收率为９９．２２％（ｎ＝３），变异系数０．５０％～０．７４％（ｎ＝５）。进样量在０．０６８μｇ～０．６８μｇ范围内线性关系良好，ｒ＝０．９９９９。所建立的方法可作为评定杜仲及杜仲制品降压效果的一种简便、快速和准确的测定方法。

A reversedphase high performance liquid chromatographic method for the determination of (+)pinoresinoldiOβDglucopyranoside in the bark of Eucommia Ulmoides was established. The airdried bark of E.Ulmoides was ground into powder and extracted continuously with methanol. The extract was evaporated under reduced pressure, then suspended in water. This suspension being subjected to column chromatography was eluted successively with water, 30%, 50% and 90% aqueous solution of ethanol. The 30% EtOH eluate was concentrated and then injected into HPLC system. The conditions of HPLC were: YWGC 18 column (250mm×46mm i.d, 10μm);28%(V/V) methanol mobile phase and UV detector at 232nm. The calibration curve was linear in the range of 0.068μg0.68μg of(+)pinoresinoldiOβDglucopyranoside with correlation coefficient of 0.9999. The coefficient of variation and average recovery were 0.50%0.74% (n=5) and 99.22% (n=3) respectively.