摘要
从５６个土样中分离获得２５３株脂肪酶产生菌，其中ＮＱ２３菌株产酶活性最高，从其形态特征确定为根霉属（Ｒｈｉｚｏｐｕｓ）。对该菌株进行紫外线和硫酸二乙酯诱变，酶活力提高５２％。对该菌株的产酶条件及部分酶性质进行了研究后发现该菌株所产脂肪酶具有良好的热稳定性，最适作用温度较高，为５０℃左右。
253 lipaseproduction strains were selected from 56 oil contained earth samples.We screened a high lipaseyielding strain named NQ 23 .This strain was identified as Rhizopus based on its characteristic and the lipase activity was increased by 52% after this strain was mutated by UV and DES.In addition,the optimum lipase producing condition and the characteristic of this strain were studied.The results show :The lipase produced by this strain has good thermostability and was stable below 70℃.Its optimum temperature was about 50℃ or higher.
基金
江苏省专项化学品计划研究课题
