摘要
目的探讨不同的冻存保护液对于胚胎大鼠嗅球神经干细胞(nerural stem cells,NSCs)生物特性的影响。方法我们应用不同冻存保护液对胚胎大鼠嗅球NSCs进行冻存,研究低温冻存以及不同的冻存保护剂对该细胞增殖及多向分化潜能的影响,分析冻存与复苏对于胚胎大鼠嗅球NSCs生物学特性(细胞活力、克隆形成率、生长曲线、分化能力)的影响。结果采取正确的冻存与复苏方法,对胚胎大鼠嗅球NSCs的生物学特性无影响。结论胚胎大鼠嗅球NSCs经低温长期冻存,复苏后对于该细胞的基本生物学特性没有明显影响,可以低温冻存3个月以上。
OBJECTIVE To observe the changes of biological feature of neural stem cells （NSCs） of embryonic olfactory bulb in rats after cryopreservation. METHODS The survival rates of cryopreserved embryonic rat olfactory bulb NSCs were compared after treated with four different cryoprotectants： dimethylsulfoxide （DMSO） , glycerol, each with or without 10% FBS and with two different storage periods in liquid nitrogen （- 196℃） , specifically 3days short- term storage and 3months long-term storage. The recovery efficiency of embryonic rat olfactory bulb NSCs were assessed after freezing and thawing by viability testing and colony-forming assay as well as immunocytochemistry under different conditions. RESULTS No significant difference in the survival rate was observed among these different cryoprotectants and different protocols. CONCLUSION Our results imply that, under optimal conditions, embryonic rat olfactory bulb NSCs might be cryopreserved for a period of more than 3 months without losing their proliferative and multipotential activities.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2009年第1期3-6,共4页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
陕西省自然科学基础研究计划资助项目(SJ08C216-13)
关键词
千细胞
低温保存
胚胎
大鼠
嗅球
Stem Cells
Cryopreservation
Embryo
Rats
Olfactory Bulb
