ASE-超高效液相色谱法快速测定烟草中的多酚类物质

Fast determination of polyphenols in tobacco by ASE-UPLC

建立了加速溶剂萃取(ASE)-超高效液相色谱(UPLC)同时测定烟草中6种多酚物质的快速方法。用50%的甲醇水溶液在50℃下对烟草样品加速溶剂萃取5min,循环萃取2次,将萃取液定容过滤后,用配置二极管阵列检测器的UPLC进行分析。此方法具有分离效率高、分析时间短、检测灵敏的优点。应用此方法对18种单料烟和14种成品卷烟中的6种多酚成分进行了测定。结果表明:①不论是在单料烟叶还是成品卷烟中,绿原酸和芸香苷的含量均占多酚总量的90%以上;②在单料烟叶中,烤烟中多酚的含量高于白肋烟和晒烟;③不同部位的烤烟中,多酚含量从上部、中部和下部依次降低,这表明多酚成分可成为烟叶等级判别和质量控制的化学指标之一;④烤烟型卷烟中多酚的含量显著高于混合型卷烟中多酚的含量。

An accelerated solvent extraction （ASE）-ultra performance liquid chromatography （UPLC） method for the fast determination of 6 polyphenols in tobacco was established. Tobacco samples were extracted by ASE in 50% methanol solution under 50℃ for 5 min twice. The extracted solution was analyzed by a UPLC equipped with diode array detector after filtration. This method has the merits of high separation performance, fast determination, and good sensitivity. Polyphenols in 18 tobacco leaves and 14 cigarette samples were detected. Results indicated that the content of chlorogenic acid and rutin accounted for above 90% in both tobacco leaves and cigarette samples. The content of polyphenols in flue-cured tobacco leaves were higher than that in burley tobacco and suncured tobacco. Polyphenols content in flue-cured tobacco leaves reduced gradually from upper to bottom, which indicates that polyphenol compounds may be used as the chemical index for the tobacco grade discrimination and quality control. In addition, polyphenols in flue-cured cigarette were evidently higher than those in blended type cigarette.