采用GC-MS测定侧流卷烟烟气中芳香胺

Determination of aromatic amines in side-stream cigarette smoke by GC/MS

芳香胺类化合物是卷烟烟气中的一类重要有害物质,测定卷烟烟气中,特别是侧流卷烟烟气中的芳香胺类化合物还没有准确可靠的方法。实验中采用鱼尾罩和装有剑桥滤片的捕集装置捕集侧流卷烟烟气中的芳香胺类化合物,在酸性条件下用二氯甲烷萃取芳香胺类化合物,用五氟丙酸酐和三甲胺在强碱性条件下,对芳香胺进行衍生,利用四级杆GC-MS测定4种芳香胺的五氟丙酸酐衍生物。实验证明,此方法有较好的重复性:对4种芳香胺类化合物测定的相对标准偏差在6%以下;具有较高的灵敏度:4种芳香胺类化合物的检出限均低于0.16ng/支;较高的准确性:4种芳香胺类化合物的回收率在94.41%～102.70%之间,完全适用于定量检测卷烟侧流烟气中芳香胺类化合物。

Aromatic amines is a possible source of carcinogenicity in cigarette smoke. Accurate determination of aromatic amines is of great help to assess the risk of cigarette to public health. A specific and sensitive method to quantitatively analyze aromatic amines in side-stream cigarette smoke was developed and validated. Cigarette smoke was collected using a Cambridge filter connected to a fishtail chimney. The collected aromatic amines were extracted with 5% hydrochloric acid solution and dichloromethane, derivatized with pentafluoropropionic acid anhydride and trimethylaroine and analyzed by GC/MS. The method is highly repeatable. The relative standard deviations were generally below 6%. Limit of detection for every aromatic amine was less than 0.16 ng/cig. The recoveries were in the range of 94.41% to 102.70% . This method was applicable for routine analysis of aromatic amines in side-stream cigarette smoke.