Ce(Ⅳ)-罗丹明6G化学发光体系快速测定染料木素

Cerium(Ⅳ)-rhodamine-6G syetem for the determination of cenistein

在硝酸介质中,Ce(SO4)2氧化染料木素,罗丹明6G可以增强其化学发光强度.据此采用流动注射技术,建立了Ce(IV)-罗丹明6G-染料木素化学发光体系测定染料木素的化学发光新方法.在优化的实验条件下,该法测定染料木素的检出限为0.4μg/L,线性范围为0.002～5.000μg/mL,对0.5μg/mL染料木素进行了11次平行测定,其RSD为1.67%.将本法用于尿样及槐角中染料木素含量的测定获得满意结果,并对反应可能的机理进行了探讨.

A chemiluminescence method for the determination of genistein was presented, which is based on its oxidation with cerium（/V） in nitric acid media accompanied with chemiluminescence using Rh6G as sensitizer, Under the optimum conditions, the linear range for the determination of genistein is in the range of 0. 002～5. 000μg/mL with a detection limit of 0.4μg/ L. The relative standard deviation for 0.5 μg/mL genstein is 1.67% （n = 11）. The proposed method has been applied to the determination of genistein in pharmaceutical preparations and u- rine samples with satisfactory results. A possible mechanism has been prospoed based on the investigation CL and UV spectra of the reaction system.