荔枝草及其混淆品的HPLC指纹图谱鉴定

Identification on Common Sage Herb and its Adulterants by HPLC Fingerprint Chromatogram

目的:建立荔枝草及其混淆品的指纹图谱鉴定方法。方法:色谱柱:Hypersil C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;柱温:40℃;检测波长:290 nm;体积流量:0.9 mL/min。结果:共标出荔枝草药材9个共有峰,荔枝草药材与混淆品的指纹图谱有明显区别。结论:HPLC指纹图谱鉴别荔枝草及其混淆品具有重复性好、特征性强、方法简便等特点,可作为荔枝草药材的鉴别方法。

Objective ： To establish the fingerprint chromatogram for the identification of Common Sage Herb and its adulterants. Methods： The separation was performed by Hypersil C18 column （4.6 mm × 250 mm,5 μm） with a mobile phase of Acetonitrile and 0.1% Phosphoric Acid mode at a flow rate of 0.9mL/min. The detection of wavelength was 290mn. Resuhs：9 peaks were separated on the HPLC fingerprint of Common Sage Herb. It was found that there were great difference in fingerprint chromatogram among them. Conclusion： This method is simple, rapid, stable and with good reproducibility. It may be used to identify Common Sage Herb.