阳离子交换树脂氯化钠溶液洗脱纯化益母草总碱的研究

The Research on the Technology of Purificating Total Alkaloids in Herba Leonuri with Sodium Chloride and Acidic Exchange Ion Resins

目的:优选阳离子交换树脂以氯化钠作为洗脱剂纯化益母草总碱的最佳工艺条件。方法:采用732型阳离子树脂,以益母草总碱和盐酸水苏碱洗脱率为指标,优选最佳纯化工艺参数。结果:以3.0mol/L氯化钠溶液洗脱时益母草总碱的洗脱率为87.35%,盐酸水苏碱的洗脱率为86.04%,经纯化所得提取物的益母草总碱含量>75%。结论:采用732型阳离子树脂以氯化钠溶液作洗脱剂纯化益母草总碱,不仅洗脱率高,成本低,而且易于推广至工业化生产。

Objective：Selecting the optimum purificating process of total alkaloids in herba leonuri with sodium chloride and acidic exchange ion resin. Method：Selecting Staehydrine Hydrochloride and total alkaloids in leonuri as index, and researching the optimum purifieating process by acidic exchange ion resin of 732 type. Result：3mol/L salt water was tested to he the best：the purification ratio of Stachydrine Hydroehloride is 86.04% ,total alkaloid is 87.35% ;the purification ratio of total alkaloid of the extract is more than 75%. Conclusion ： The technology of salt water eluting alkaloids in herba leonuri by acidic exchange ion resin of 732 type can overcome the defects such as low elution ratio, high cost, and unsuitable for industrial production.