摘要
[目的]探讨姜黄素(curcumin,CUR)对大鼠肝纤维化的防治作用及对肝组织bax和bcl-2蛋白及基因表达的影响。[方法]建立CCl4致大鼠实验性肝纤维化模型。用免疫组化法比较正常组、模型组以及CUR组的bcl-2、bax蛋白的表达,用RT-PCR法比较各组肝组织bcl-2、bax mRNA的表达。[结果]CUR组肝组织炎症和纤维化程度较模型组显著减轻。bax蛋白主要表达在肝细胞质,而纤维间隔及汇管区呈低表达;bcl-2主要表达在纤维间隔及汇管区。bax及bcl-2在模型组及CUR组表达均高于正常组(P<0.01),CUR组均较模型组降低(P<0.01)。bax及bcl-2 mRNA在模型组及CUR组表达比正常组增高(P<0.01,<0.05),CUR组均较模型组降低(P<0.05)。[结论]CUR可能通过降低肝细胞表达bax、减少肝细胞凋亡而减轻肝脏损伤,通过降低bcl-2表达、诱导肝星状细胞凋亡发挥减轻肝纤维化的作用,防治大鼠肝纤维化。
[Objective]To evaluate the expression of bax/bcl-2 in rat with liver fibrosis and the influence of curcumin. [Methods] Liver fibrosis in rats were induced by carbon tetrachloride （CCl4）. Rats were divided into three groups, normal control group , CCl4 model group and curcumin group. The protein expression of bax/bcl-2 were detected by immunohistochemistry. The mRNA expression of bax/bcl-2 were determined by RT-PCR. [Results] Compared with model group, curcumin could considerably attenuate the extent of liver inflammation and fibrosis. Rat hepatic fibrosis developed as showed by histological examination. The degree of hepatic fibrosis in curcumin group alleviated obviously compared to CCL4 model group. Both immunohistochemistry and RT-PCR showed, the expression of bax increased markedly in curcumin group and CCl4 model group than normal control group. Bcl-2 expression was also up-regulated than normal control group as well the expression of bax/ bcl-2 was lower in curcumin group than CCl4 model group. [Conelusion ] Curcumin can effectively prevent and treat liver fibrosis through inhibiting the expression of the promoting apoptosis gene hax and restraining apoptosis gene bcl-2.
出处
《中国中西医结合消化杂志》
CAS
2008年第6期362-365,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
江苏省中医药管理局资助(H05096)
