左金丸及拆方对胃溃疡大鼠胃黏膜愈合和表皮生长因子受体表达的影响

Therapeutic effects of Zuojinwan and its ingredients on rat gastric ulcer induced by acetic acid and influence on EGFR expression of the gastric mucous

[目的]探讨左金丸及其拆方对胃溃疡(GU)愈合的影响、机制及配伍依据。[方法]建立乙酸性GU大鼠模型,54只动物随机均分成模型组、黄连组、吴茱萸组、左金丸组,并设立空白组和假手术组,每组9只,观测各组大鼠溃疡指数(UI)和表皮生长因子受体(EGFR)的表达。[结果]①黄连组、吴茱萸组、左金丸组UI显著低于模型组(P<0.01),且依次降低(P<0.01,<0.05);②溃疡形成后模型组GU边缘EGFR表达显著高于空白组(P<0.01),左金丸组显著高于模型组(P<0.01),各治疗组间比较差异无统计学意义。[结论]左金丸及其拆方均有促进溃疡愈合的作用。显著性上调EGFR的表达可能是左金丸促进溃疡愈合的重要机制之一。

[Objective]To investigate the therapeutic effects and the mechanism of Zuojinwan and its ingredients on rat gastric ulcer. [Methods]SD rats with gastric ulcer induced by acetic acid were randomly divided into model group, Coptis clinensis group, Evodia rutaecarpa group and Zuojinwan Group. And normal group and sham-operation group were set, each group had 9 rats. Ulcer index（UI） and the epidermal growth factor receptor （EGFR） expression of each group were observed. [Results] （1） Compared with the model group, the UI in Coptis clinensis group, Evodia rutaecarpa group and Zuojinwan Group decreased（P〈0. 01）. （2）Contrast to the normal group, the EGFR expression of the gastric mucous in the model group were significantly enhanced （P〈0. 01）. And that of the Zuojinwan group increased comparing to the model group（P〈0. 01）. There was no significant difference of EGFR expression among the three treatmental group. [Conclusion ]Coptis clinensis,Evodia rutaecarpa and Zuojinwan are effective in treating rat gastric ul- cer induced by acetic acid and Zuojinwan may treat gastric ulcer by significantly improving the level of EGFR.