重组干酪乳杆菌在模拟动物胃肠道内的稳定性检测

Stability Examination of Recombinant Lactobacillus Casei in Imitative Gastroenteric Environments

为确定本实验室研究构建的表达猪传染性胃肠炎病毒S蛋白重组干酪乳杆菌在模拟动物胃肠道内的稳定性,在人工模拟胃肠道环境中,即模拟人工胃液、人工肠液和胆汁环境下进行活菌耐受力检测。结果表明,重组菌能够在含有胃蛋白酶pH1.5的盐酸存活2h和在pH为2.5的盐酸耐受性良好,能够耐受胰蛋白酶溶液和0.3%的胆汁。

In order to define the stability of the recombinant strain Lactobacillus casei expressingporcine transmissible gastroenteritis virus S protein in imitating gastroenteric environments, the cultivate conditions,the stability of the recombinant plasmids were detected. The results indicated that the recombinant strain could survived in hydrochloric acid included pepsin for 2 hours of pH1.5 and stand well of over pH2.5, bear and survive in imitative gastroenteric environments of trypsin and the concentration of 0.3% bile.