聚四氟乙烯纤维束膜外壁附牛血清蛋白吸附胆红素的初步研究

The adsorption of bilirubin by PTFE fiber membrane attached with BSA on its outer layer:Apreliminary experimental study

目的探索使用生物相容性良好的聚四氟乙烯(PTFE)中空纤维管,并在其外壁附载牛血清白蛋白(BSA)制成一次性血浆滤过器.寻求一种安全有效、操作方便、费用合理的血浆净化清除胆红素方式。方法胆红素吸附试验选取甲基丙烯酸环氧丙酯(GMA)接枝率为35%,每克MPTFE纤维附载BSA为75mg,用离心浇注法制成血浆滤过器,滤过器面积约为0.35m2。避光,恒温37℃,流速150～250ml/min。血浆在纤维管内循环,利用纤维管内外胆红素浓度梯度差,血浆中与白蛋白结合的胆红素解离扩散通过膜孔并与管壁外附着的BSA再结合,使滤器中胆红素浓度达到平衡;观察血浆中胆红素浓度随时间的变化。结果血浆中胆红素浓度随透析时间的延长明显降低;在100min后趋于平衡,吸附率约为15.26%～32.11%。结论MPTFE中空纤维管外壁修饰牛血清白蛋白(BSA)滤过吸附胆红素方法对血浆中胆红素有明显降低作用。由于吸附过程不与血浆直接接触,相对于分子吸附再循环(MARS)系统[1]简单,相比血浆灌流吸附解毒方式[2]更安全。

Objectives To study the effect of PTFE hollow fiber tube attached with bovine serum albumin （BSA） on its outer layer on the adsorption of bilirubin. Methods For bilirubin adsorption test, we selected glytidy methacrylate （GMA） of 35% grafted rate and attached with BSA 75 mg per gram fiber MPTFE, centrifugal casting of the legal system into a plasma filter, having about 0.35m^2 of filter area, and under the conditions of dark, 37℃ and 150-250ml/min plasma flow rate. Plasma circulated in the fiber tube. Due to the concentration gradient of bilirubin exterior and interior of PTFE tube, bound bilirubin in plasma dissociates from albumin, passes through the fenestrated membrane, and rebinds the BSA on the outer wall of the membrane, resulting in a new balanced bilirubin concentration across the membrane. We also observed the changes of plasma bilirubin concentration along with time. Results After the dialysis, plasma bilirubin concentration decreased gradually and significantly, reached the balanced concentration after 100 minutes with the bilirubin adsorption rate of about 15.26%-32.11%. Conclusion The PTFE hollow fiber tube attached with bovine serum albumin （BSA） on its outer layer filters and adsorbs plasma bilirubin in the tube, resulting in the decrease of plasma bilirubin. The treated plasma is isolated from other plasma, and this method is therefore simpler than that using molecular absorption of recycling system （MARS）. Dialysis using this tube is safer than the detoxication and adsorption procedure using plasma perfusion.