肺炎链球菌感染儿童血浆甘露糖结合凝集素水平分析

Analysis of Mannose-binding lectin plasma concentration in children with infection caused by Streptococcus pneumoniae

目的了解肺炎链球菌感染儿童血浆甘露糖结合凝集素表达水平的变化,比较健康组和肺炎链球菌感染组血浆MBL浓度的差异。方法血浆MBL浓度的检测采用ELISA法。统计分析采用SPSS软件11.0版。不同人群间MBL浓度比较采用非参数检验。结果MBL浓度分布为非正态分布资料,105例健康儿童血浆MBL浓度范围为3 ng/ml-6,025 ng/ml和49例肺炎链球菌感染儿童血浆MBL浓度范围为0-6,544 ng/ml,中位数分别为1,057ng/ml和1,307 ng/ml,肺炎链球菌感染儿童血浆MBL浓度明显高于健康儿(Z(=2.236,P=0.025)。健康组和感染组血浆MBL浓度低于200 ng/ml的个体分别占5.71%和10.2%,两者间差异无统计学意义(χ2=1.02,P>0.05)。结论本研究未能证明肺炎链球菌的易感性与血浆MBL浓度的相关性。

Objective To understand the change of Mannose-binding lectin （MBL） plasma concentration in children with infection caused by Streptococcus pnenmoniae, and to compare the difference of MBL plasma concentrations between healthy children and children infected by Streptococcus pneumoniae. Methods MBL plasma concentrations were measured by ELISA method with human MBL ELISA kit, and the MBL levels between two groups were analyzed with nonparametrie test by SPSS 11.0 （statistics package for social science）. Results Data of MBL plasma concentrations were not normal distribution. The values of MBL plasma concentrations in healthy children（ 105 case） ranged from 3 ng/ml to 6 025 ng/ml and the median was 1 057 ng/ml, and the values in children with infection caused by Streptococcus pneumoniae （49 case） ranged from 0 to 6 544 ng/ml and the median was 1 307 ng/ml. Mannose-binding lectin（MBL） plasma concentration in children with infection caused by Streptococcus pneumoniae was significantly higher than that in the healthy children. 5. 71% of the healthy children and 10.2% of children with infection children caused by Streptococcus pneumoniae had low MBL levels whose concentrations were less than 200 ng/ml, however, no significant difference was found between the two groups（Х^2 = 1.02, P 〉 0.05）. Conclusion The research can not prove the correlation between the sensitivity to Streptococcus pneumoniae and Mannose-binding lectin （MBL） plasma concentration.