定心胶囊对房颤大鼠血栓素B_2及基质金属蛋白酶9的影响

Effect of Dingxin Capsule on TXB_2 and MMP-9 Level in Rats with Atrial Fibrillation

目的观察定心胶囊对房颤大鼠血栓素B2(TXB2)、基质金属蛋白酶9(MMP-9)的影响,探讨其预防房颤的作用机理。方法采用氯化钙-乙酰胆碱混合液以鼠尾静脉给药的方式建立房颤的动物模型。筛选典型房颤心电图表现的大鼠,随机分为房颤模型组、定心胶囊治疗组、维拉帕米对照组及正常组。定心胶囊治疗组、维拉帕米对照组给药4周后,测定血清中TXB2、MMP-9的含量。结果定心胶囊治疗组与房颤模型组比较,血清中TXB2、MMP-9的含量明显降低,均P<0.05,差异有统计学意义;定心胶囊治疗组与西药对照组比较,房颤大鼠血清中TXB2、MMP-9的含量均降低,且定心胶囊治疗组优于西药对照组。结论定心胶囊能降低血清中TXB、MMP-9的含量,是防治房颤的有效药物。

Objective To research the effect of Dingxin capsule on the level of TXB2 and MMP-9 in rats, and explore the mechanism of treating and preventing atrial fibrillation （AF） by TCM. Method AF animal model was established by injecting the calcium chloride-acetylcholine mixed liquid in coccygeal vein of rats The rats with typical ECG were divided randomly into 4 groups. AF model group （M-group）, Dingxin capsule group （D-group）, verapamil group （V-group）, normal group （N-group）. TXB2 and MMP-9 were measured 4 weeks later. Result Comparing with M-group, the level of TXB2 and MMP-9 of D-group decreased obviously （P〈0.05）. TXB2 and MMP-9 of both V-group and D-group decreased, while D-group was better, with statistical significance. Conclusion Dingxin capsule can reduce TXB2 and MMP-9 in serum, and is effective in treating and preventing atrial fibrillation.