蛋氨酸硒对西门塔尔牛瘤胃发酵及尿嘌呤衍生物的影响

Effect of selenium methionine on rumen fermentation and purine derivatives in Simmental steer

选用4头平均体重420kg、年龄2.5岁装有永久性瘤胃瘘管的西门塔尔牛阉牛,采用4×4拉丁方设计,以混合精料和风干玉米秸秆为基础日粮,以蛋氨酸硒为硒源,在基础日粮中分别添加硒0mg·kg-1、0.3mg·kg-1、0.6mg·kg-1和0.9mg·kg-1,研究蛋氨酸硒对西门塔尔牛瘤胃pH、氨态氮、VFA、营养物质降解率及尿嘌呤衍生物浓度的影响。结果表明:不同浓度硒处理间西门塔尔牛瘤胃pH、挥发性脂肪酸(VFA)和乙酸/丙酸无显著差异(P>0.05),氨态氮浓度显著低于对照(P<0.05);0.6mg·kg-1硒处理组西门塔尔牛玉米秸秆干物质(DM)、有机物质(OM)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和豆粕DM、OM、粗蛋白质(CP)有效降解率均显著高于对照(P<0.05);0.6mg·kg-1和0.9mg·kg-1硒处理组西门塔尔牛尿囊素和尿嘌呤衍生物浓度显著高于0.3mg·kg-1组和对照组(P<0.05);西门塔尔牛日粮以蛋氨酸硒为硒源时,硒的适宜添加浓度为0.6mg·kg-1。

Four Simmental steers （average BW 420 kg, aged 2.5 years） cannulated in rumen consuming corn straw diet were used in 4x4 Latin square design to evaluate the effects of different selenium methionine on ruminal pH, ammonia, volatile fatty acid, nutrient degradability and purine derivative of urine. Treatments consisted of 0 mg （control）, 0.3 mg, 0.6 mg and 0.9 mg supplemental Se （selenium methionine） per kg DM diet. The results show that ruminal pH, volatile fatty acid concentration and actate to propionate ratio are not affected （P 〉 0.05）. Ammonia concentrations in supplemented selenium methionine groups are lower than the control （P 〈 0.05）. Degradability of dry matter, organic matter, and neutral and acid detergent fiber of corn straw, and the degradability of dry matter, organic matter and crude protein of soybean in the 0.6 mg supplemental Se per kg DM are significantly higher than the control group （P 〈 0.05）. Allantoin and purine derivatives in group supplemented with 0.6 mg and 0.9 mg Se per kg are significantly higher than both the control and the 0.3 mg supplemental Se per kg DM （P 〈 0.05）. Supplementation of selenium methionine has passive effect on rumen fermentation. Optimum Se supplementation dose is 0.6 mg·kg^-1.