Bmi-1在正常胃组织和胃癌及其癌前病变中的表达及意义

The Expression of Bmi-1 Protein in Normal Gastric Mucosa,Gastric Carcinoma,and Precancerous Lesions

目的：检测Bmi-1在正常胃粘膜、胃癌及癌前病变中的表达，分析探讨Bmi—1与核增殖抗原ki-67和凋亡抑制基因Bcl-2之间的关系，以及Bmi-1^＋细胞与Bcl-2^＋／ki-67^-细胞分布之间的关系，探讨其与胃癌临床病理因素的关系及意义。方法：采用免疫组化Envision法检测162例胃癌及配对正常胃粘膜中Bmi-1和ki-67的表达，采用免疫组化双染法观察Bcl-2^＋／ki-67^-细胞的分布。结果：胃癌组织中Bmi-1表达（52．5％）明显高于正常胃粘膜（21．6％），P〈0．05；胃癌中Bmi-1表达与Lauren分型、Borrmann分型和胃癌临床病理分期有关（P〈0．05）。胃癌中ki-67表达与Borrmann分型有关（P〈0．05）。Bcl-2在胃癌中的表达与胃癌Lauren分型有关（P〈0．05）。在胃癌组织和肠化生粘膜中，Bmi-1与Bcl-2呈正相关（P〈0．05）。结论：胃癌及其癌前病变中Bmi-1表达可能与细胞增殖、凋亡和癌变有关。在胃癌和肠化生粘膜中Bmi-1和Bcl-2表达的相关性以及Bmi-1^＋细胞和Bcl-2^＋／ki-67^-细胞分布之间的关系提示Bmi-1与胃癌的发生密切相关。

Objective： To investigate the expression of Bmi-1 in normal gastric mucosa, gastric carcinoma and precancerous lesions; to analyze the correlation of Bmi-1, ki-67 and Bcl-2 with the distribution of Bmi-1（＋） cells and Bcl-2 （＋）/ki-67 （-） cells; and to explore the correlation between Bmi-1 expression with clinicopathologic features of gastric carcinoma. Methods： Envision immunohistochemistry and immunohistochemical double-labelling staining were used to detect the expression of Bmi-1, ki-67, Bcl-2 and the distribution of Bcl-2 （＋） /ki-67 （-） cells in 162 gastric carcinoma specimens. Results： The expression of Bmi-1 in gastric carcinoma specimens （52.5%） was significantly higher than in normal gastric mucosa （21.6%） （P〈0.05）. The expression of Bmi-1 was related to Lauren＇s classification, Borrmann classification and clinical pathological stage （P〈 0.05）. The expression of ki-67 was related to Borrmann classification in gastric carcinoma （P〈0.05）. The expression of Bcl-2 was related to Lauren＇s classification （P〈0.05）. In gastric carcinoma and intestinal metaplasia tissues, Bmi-1 expression was positively correlated with Bcl-2 （P〈0.05）. Conclusion： The variation of Bmi-1 expression in gastric carcinoma and precancerous lesions might be involved in cell proliferation, apoptosis and cancerization. In gastric cancer and intestinal metaplasia, Bmi-1 is significantly associated with progression of gastric carcinoma.