纤维粘连蛋白基因多态性与尘肺易感性的研究

Relationship between gene polymorphism of fibronectin and pneumoconiosis

目的探讨纤维粘连蛋白（FN）4个基因位点多态性与尘肺发病的关系。方法以确诊的128例汉族男性Ⅰ期尘肺患者作为病例组，选择与病例组年龄相差不超过5岁、同性别、同民族、开始接尘时间及累积接尘工龄相差不超过2年、在同一车间或同一采掘工作面工作的非尘肺接尘工人为对照。采用聚合酶链反应-限制性片断长度多态性分析（PCR-RLFP）的方法检测FN基因HaeⅢb、MspⅠ、HindⅢ、TaqⅠb位点的基因型。结果病例组携带MspⅠ野生基因型（CC）频率和等位基C的分布频率分别为10．9％和41．8％，明显高于对照组（分别为3．9％和31．2％），差异有统计学意义（P〈0．05）。病例组携带HaeⅢb野生基因型（AA）的频率为24．2％，明显高于对照组（17．9％），差异有统计学意义（P〈0．05）；病例组HaeⅢbA等位基因频率为51．9％，明显高于对照组（42．2％），差异有统计学意义（P〈0．05）。病例组和对照组TaqⅠb位点基因型和HindⅢ位点基因型的分布频率比较，差异均无统计学意义（P〉0．05）。病例组同时携带MspⅠCC和HaeⅢbAA基因型的频率明显高于对照组，差异有统计学意义（P〈0．05）。结论携带FN MspⅠCC和HaeⅢbAA基因型的接尘工人患尘肺的危险性增加，同时携带这2种基因型的接尘工人更易患尘肺。未发现FN TaqⅠb和HindⅢ位点基因多态性与尘肺易感性有关。

Objective To explore the relationship between the genetic polymorphism of fibronectin （FN） （4 genetic locus） and pneumoconiosis. Methods 128 male Ⅰ-period pneumoeoniosis were selected as cases who were examined with radiography and diagnosed by the Pneumoconiosis Diagnosis Expert Panel, based on the Chinese National Diagnosis Criteria of Pneumoconiosis （GBZ 70-2002 ）. According to 1：1 paired matching method, 128 dust exposure workers were selected as control who were exposed to same dust as cases. The difference of age and cumulative length of service between case and control was not over five years and two years, respectively. 5 ml peripheral venous blood was drawn and anticoagulated with 2% EDTA. The polymorphisms of FN （Msp Ⅰ ,Taq Ⅰ b, Hind Ⅲ, Hae Ⅲ b） were detected, using the method of polymerase chain reactionrestriction fragment length polymorphism （PCR-RFLP） techniques and PCR. Results The frequencies of FN Msp Ⅰ （CC） in cases and control groups were 10.9% and 3.9%,respectively. The difference was significant （P〈0.05）. The frequencies of FN （Msp Ⅰ ）C allele were 41.8% and 31.2% in case and control,and the difference between cases and controls was significant （P〈0.05）. The frequencies of FN Hae Ⅲ b （AA） genotype in cases （ 24.2% ） was higher than that in control groups （ 17.9% ）, OR =5.0 （ 95%CI： 4.840-24.210）. The frequencies of FN（ Hae Ⅲ b ）A allele were 51.9% and 42.2% in case and control, and the difference was significant（P〈 0.05 ）. The difference of Taq Ⅰ b and Hind Ⅲ genotype between cases and controls were not significant（P〉 0.05 ）. Conclusion The risk of suffering from pneumoconiosis increases in workers canting FN （ Msp Ⅰ CC or Hae Ⅲb AA ） genotype after exposure to dust. Workers both canting FN （ Hae Ⅲ b AA ） and （ Msp Ⅰ CC ） genotypes are more susceptible to pneumoeoniosis. The relationship between genetic polymorphism of FN（Taq Ⅱb, Hind Ⅲ ） and pneumoconiosis has not been found.