云南个旧和宣威地区肺癌hTERT mRNA与Mad1蛋白表达的研究

Expression of hTERT and Mad1 in lung cancer in Gejiu and Xuanwei of Yunnan Province

目的研究hTERT mRNA和Mad1蛋白在个旧地区矿工肺癌及宣威地区农民肺癌组织中的表达，探讨hTERT mRNA和Mad1蛋白的相互关系。方法采用免疫组化S-P法，分别检测40例个旧地区矿工肺癌（个旧矿工肺癌组）和20例宣威地区农民肺癌（宣威农民肺癌组）以及20例正常肺组织（正常肺组织组）中Mad1蛋白的表达；采用原位杂交方法检测上述标本中hTERT mRNA的表达情况。染色阳性结果应用计算机图文分析系统进行定量分析。结果个旧矿工肺癌组和宣威农民肺癌组Mad1蛋白表达的阳性单位分别为16．77±6．01，19．36±4．54，与正常肺组织组（46．05±7．26）比较明显降低，差异有统计学意义（P〈0．01）。个旧肺癌组、宣威肺癌组Mad1蛋白表达的阳性率明显低于正常肺组织，差异有统计学意义（P〈0．01）。个旧矿工肺癌组hTERT mRNA表达的阳性单位为（72．10±13．07），宣威农民肺癌组为（74．20±15．17），明显高于正常肺组织组（10．70±2．21），差异有统计学意义（P〈0．01）。个旧肺癌组、宣威肺癌组hTERT mRNA表达阳性率明显高于正常肺组织，差异有统计学意义（P〈0．01）。hTERT mRNA和Mad1蛋白表达的阳性单位和阳性率均呈负相关关系（r^1=0．9881、r^2=0．999，P〈0．05）。结论hTERT mRNA在肺癌的发生发展中可能起重要作用，hTERT mRNA表达上调和Mad1蛋白表达缺失与肺癌的发生密切相关。

Objective To study the expression of hTERT mRNA and Mad1 protein in lung cancer of Gejiu and Xuanwei and normal lung tissue and to investigate their correlations with lung cancer. Methods Mad1 protein was detected by immunohistochemistry S-P method, and hTERT message RNA （mRNA） was detected by in situ hybridization （ ISH ） in 40 specimens of lung cancer of Gejiu Tin miners and 20 specimens of lung cancer of Xuanwei peasants and 20 specimens of normal lung tissue. The positive signals were quantitatively analyzed by HPIAS- 100. Results The positive unit （PU） of Mad 1 protein was 16.77±6.01 in Gejiu Tin Miners lung cancer group, and 19.36±4.54 in Xuanwei peasant lung cancer group,compared with the normal lung tissue （46.05±7.26）. The difference was highly significant（P〈0.01 ）;The PU of hTERTmRNA was 72.10±13.07 in Gejiu Tin miners lung cancer group,and 74.20±15.17 in Xuanwei peasant lung cancer group,which was higher than that in normal tissue group（ 10.70±2.21 ）. The difference was significant（P〈0.01 ）. The expression of Mad1 protein was negatively correlated with the expression hTERTmRNA （P〈0.05,r=-0.9881, r=-0.999）. Conclusion Reduced hTERT mRNA expression may play an important role in the occurrence of lung cancer. The expression of hTERT mRNA and deletion of Mad1 protein are closely related to pathogenesis of lung cancer.