摘要
以253头中国荷斯坦奶牛为研究对象,检测HSP70-1基因的多态性,并分析其多态性与中国荷斯坦牛体细胞评分(Somatic cell score,SCS)的相关性。首先以PCR-SSCP法寻找HSP70-1基因编码区的突变,并通过测序确定突变的类型,根据突变类型寻找合适的内切酶,最终采用PCR-RFLP方法鉴定实验牛基因型;然后分析基因多态性与中国荷斯坦牛SCS的相关性。结果表明HSP70-1基因的1623bp处产生G→A→C突变,2409bp处产生G→A突变,两位点都是沉默突变,未引起氨基酸序列的改变;经χ2适合性检验,中国荷斯坦牛在两个位点均未达到Hardy-Weinberg平衡状态;同时,群体基因座不同基因型与SCS相关分析的结果表明,2409位点基因型与SCS相关性不显著(P>0.05),1623位点基因型与SCS相关性显著(P<0.05),CC型SCS显著低于AG、GG型(P<0.05),CC基因型为乳腺炎抗性基因型。在中国荷斯坦奶牛群体中,HSP70-1基因CC基因型可作为改良奶牛乳腺炎抗性性状的分子遗传标记。
The polymorphisms of HSP70-1 gene in 253 Chinese Holstein dairy cows were studied, and the association between the polymorphisms and somatic cell score （SCS） were analyzed. PCR-SSCP, PCR-RFLP and DNA sequencing were used to investigate mutations in the coding region of HSP70-1 gene. The G→A→C mutation at 1 623 bp and G→A mutation at 2 409 bp were found and both of them were silence mutations that caused no alteration in amino acid sequence. Chi-square test Showed both loci weren＇t at Hardy-Weinberg disequilibrium in Chinese Holstein. In the meanwhile, the association of 2 409 locus and SCS was not significant. However, the polymorphism at 1623 locus affected SCS significantly （P〈0.05）. The SCS of genotype CC was significantly lower than that of genotype AG and GG （P〈0.05）, so genotype CC was mastitis resistant. These results suggest that genotype CC of HSP70-1 gene may be used as a molecular and genetic marker to improve the phenotype of anti-mastiffs in Chinese Holstein dairy cows.
出处
《遗传》
CAS
CSCD
北大核心
2009年第2期169-174,共6页
Hereditas（Beijing)
基金
国家高技术研究发展计划项目(863计划)(编号:2006AA10Z1D9,2007AA10Z169)
山东省农业科学院高技术创新基金项目(编号:2006YCX028)资助
