摘要
根据FGF5基因已知DNA序列设计了2对引物,采用PCR-SSCP和PCR-RFLP技术,在内蒙古绒山羊群体中进行基因多态性检测,结果发现FGF5基因外显子1存在限制性内切酶BglⅠ多态位点。对其不同基因型个体PCR回收产物进行测序,测序结果发现该SNP是由碱基序列C→T的突变而引起的。基因型和基因频率统计,该实验群体以等位基因A具有明显的优势,χ2检验表明该SNP位点的基因频率处于Hardy-Weinberg平衡状态;对该SNP与绒毛性状关联分析,表明该SNP对绒纤维伸直长度(P<0.01)和含绒量(P<0.05)有显著影响,而对其他各绒毛性状的影响不显著(P>0.05)。AB基因型个体绒纤维伸直长度(P<0.01)和含绒量(P<0.05)显著高于AA基因型个体。
Two pairs primers were designed base on the known sequence for amplification of FGF5 gene in the Inner Mongolian cashmere goats herd. It was found that a restrict fragment length polymorphism within the exon 1 of FGF5 gene from the second pair of primers using PCR-SSCP and PCR-RFLP. PCR products after recovered and purified, were sequenced that randomly selected from different genotypes. It was revealed a synonymous single base mutation （C→T） for the AB genotype. The allele A was found to be predominant in Inner Mongolian cashmere goats and the allele and genotype frequencies in our sample coincident with the Hardy-Weinberg equilibrium. When analyzed for the association with fibre traits, this FGF5 SNP was found to have significant effect on cashmere fibre stretched length （P〈0.01） and cashmere rate（P〈0.05）, but have slightly effect on others fibre traits （P〉0.05）. Cashmere fibre stretched length （P〈0.01） and cashmere rate （P〈0.05） in individuals of genotype AB was significantly higher than that in individuals of genotype AA.
出处
《遗传》
CAS
CSCD
北大核心
2009年第2期175-179,共5页
Hereditas（Beijing)
基金
国家“十一五”重大科技攻关项目(编号:2006BAD01A11)
沈阳农业大学青年基金资助项目(编号:20070207)资助
