血管性血友病因子体外对胃癌细胞BGC-823增殖和黏附的影响

Effect of Von Willebrand Factor on Proliferation and Adhesion of Human Gastric Cancer Cell Line BGC-823 in Vitro

目的研究血管性血友病因子(von Willebrand factor,vWF)对胃癌细胞BGC-823增殖和黏附的影响,并初步探讨相关机制。方法体外培养人胃癌细胞株BGC-823,以vWF抗体(vWF Ab)处理;噻唑蓝比色(MTT)法检测细胞增殖活性和细胞黏附能力的变化;用倒置显微镜观察细胞形态学变化。结果vWF Ab能够抑制BGC-823细胞的增殖,抑制作用呈剂量及时间依赖性;细胞形态学观察vWF Ab作用对细胞生长具有明显的细胞毒作用;MTT黏附实验结果显示vWF Ab处理后细胞的黏附率较对照组明显下降(P<0.05),Ⅳ型胶原高浓度组较低浓度组细胞黏附率增大(P<0.01)。结论vWF在体外能够影响胃癌细胞BGC-823的增殖,并诱导细胞形态改变,促进细胞与细胞外基质的黏附。

Objective To investigate the effect on proliferation and adhesion of vWF in human gastric cancer cell line BGC 823 in vitro, and to clarify its probable mechanism. Methods Human gastric cancer cell line BGC-823 cultured in vitro was treated with vWF antibody （vWF Ab）. The effect of vWF on the proliferation and adhesion of BGC-823 in vitro was detected respectively by MTT colorimetric assay. The cell morphological changes were observed with inverted microscope. Results vWF Ab inhibited the pro liferation of BGC-823 in a time-and eoneentratiowdependent manner. Significant cytotoxicity of vWF Ab on BGC-823 cells was observed by cell morphology. Compared with the control group, the adhesion abili ty of the BGC-823 cells was decreased significantly （P〈0.01） after treatment with vWF antibody. Conclusion vWF has significant effect on the proliferation of BGC-823 cells and the cell morphological change in vitro, vWF takes an imDortant part in the Drogress of adhesion on the BGC 823 cells.