摘要
目的研究siRNA MDM2对人前列腺癌细胞PC3的MDM2表达和细胞增殖的作用。方法构建PGCsilencerTM-MDM2 siRNA,并转染入PC3细胞,分别用RT-PCR和Western blot检测siMDM2对PC3细胞MDM2基因和蛋白表达的抑制作用;用MTT法检测siMDM2对PC3增殖抑制的作用,用流式细胞术检测siMDM2对PC3细胞凋亡的影响。结果PT-PCR和Western blot结果显示siMDM2能显著抑制PC3细胞MDM2基因和蛋白的表达,抑制率最高可达65%;MTT和流式细胞术结果证明siMDM2能显著抑制PC3细胞增殖并诱导细胞凋亡。结论siMDM2能特异有效地抑制MDM2在PC3中的表达,并抑制PC3细胞增殖,促进其凋亡。
Objective To study the effect of siRNA to MDM2 on cell proliferation and MDM2 expression in prostate the cancer cell PC3. Methods PGCsilencerTM-MDM2 siRNA was constructed and transfected into the PC3 cells. MDM2 gene expression was detected by RT-PCR and protein expression was analyzed using western blot. The inhibitory effect on cell proliferation was determined by MTT assay. The cell apoptosis was observed by flow cytometer. Results The results of RT-PCR and western blot showed that the expression of MDM2 was inhibited in the siRNA transfected group and the highest inhibitory rate was 65%. The results of MTT and flow cytometer showed that siRNA could suppress the proliferation and induce apoptosis of PC3 cells. Conclusion siRNA of MDM2 could significantly inhibit MDM2 expression, the proliferation of PC3 cells and induce apoptosis of PC3 ceils.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2009年第1期24-27,共4页
Cancer Research on Prevention and Treatment
基金
吉林省科委社会发展资助项目(200505118)
