脑益康对D-半乳糖损伤的海马神经元的保护作用

Protective effect of naoyikang-containing serum on cultured hippocampus neuron injury induced by D-galactose

目的:观察脑益康对D-半乳糖(D-gal)诱导的海马神经元损伤的作用。方法:制备脑益康含药血清。应用D-gal诱导海马神经元损伤,MTT法测定细胞活力,生化法测定细胞单胺氧化酶-B(MAO-B)、Na+-K+-ATP酶和Ca2+-ATP酶活力,RT-PCR法检测细胞bax和bcl-xlmRNA的表达。结果:脑益康含药血清可显著提高细胞活力和ATP酶活力,降低MAO-B活力,上调bcl-xl mRNA的表达,降低bax mRNA的表达。结论:脑益康可通过提高ATP酶活力,降低MAO-B活力,抑制细胞凋亡,对D-gal处理的海马神经元起到保护作用。

AIM： To observe the protective effect of naoyikang - containing serum on cultured hippocampus neuron injury induced by D - galactose （ D - gal）. METHODS ： Naoyikang - containing serum was prepared in rats administered with aqueous extract of naoyikang （6.84 g · kg^-1·d^-1 ）. MTT, MAO - B and ATPase assay were used to measure the viability of hippocampus neurons. RESULTS ： D - gal at concentration of 100 μmol/L caused significant decrease in the viability of hippocampus neurons 24 h after the treatment. Naoyikang - containing serum increased the viability, ATPase activity and the expression of bcl - xl mRNA, decreased the MAO - B activity and the expression of bax mRNA in D - gal injured hippocampus neurons. CONCLUSION ： Naoyikang - containing serum prevents hippocampus neurons from D- gal induced cytotoxicity.