IFN-α 2b对人脉络膜黑色素瘤细胞的毒性作用及对端粒酶活性的影响

Effects of IFN-α 2b on telomerase activity and the toxic effects to cultured choroidal melanoma of human eyes

目的:研究干扰素(IFN-α2b)对人眼脉络膜黑色素瘤细胞端粒酶活性的影响及其对细胞的毒性作用,为临床化疗提供更多依据。方法:采用原代培养的人眼脉络膜黑色素瘤细胞做实验材料,用不同浓度IFN-α2b、作用不同时间,设立对照组,用MTT法检测IFN-α2b对细胞的毒性作用,PCR-ELISA法测定细胞中端粒酶活性的变化,并对两者关系进行相关分析。结果:随着IFN-α2b浓度及作用时间的增加,细胞中端粒酶活性逐渐下降,在药物浓度大于5×104IU/L及作用时间达到24 h后,其活性出现大幅度下降,随后出现细胞抑制率的明显升高,与端粒酶活性的下降具有一定的相关性。但均发生不同程度的滞后现象。结论:IFN-α2b可作为一种端粒酶抑制剂,有效降低体外培养的人眼脉络膜黑色素瘤细胞的端粒酶活性,呈浓度和时间依赖性,并造成细胞死亡。

AIM： To study the effects of interferon-α 2b （ IFN-α 2b） on the telomerase activity of choroidal melanoma cells of human eyes and the toxic effects to the cells. METHODS ： IFN-α 2b was used with different concentrations and times to act on the primary cultured choroidal melanoma cells of human eyes. The toxic effects were evaluated by MTT assay and the levels of telomera activity were detected by PCR - ELISA assay. A correlation between the two results was analyzed. RESULTS： The telomerase activity gradually decreased following the increasing concentrations and times of treatment with IFN -α 2b, which accompany the step - up of restrain rate of the cells. At the point when the concentration attained 5 × 10^4 IU/L or the time attained 24 h, the telomerase activity decreased very obviously. However, the appearance of cell death lagged behind the decreasing of telomerase. CONCLUSION： IFN -α 2b is an effective telomerase inhibitor, which can decrease the telomerase activity in choroidal melanoma cells of human eyes effectively. The IFN-α 2b treatment is concentration and time dependent, and can cause the death of cultured cells.