柚皮素对大鼠眼血流和脉络膜新生血管的作用(英文)

Effects of naringenin on ocular blood flow and choroidal neovascularization in experimental animals

目的:研究柚皮素对激光诱发大鼠脉络膜新生血管形成(CNV)、高眼压兔眼血流和缺血大鼠眼视网膜功能恢复的作用。方法:选择雄性棕色挪威大鼠,采用激光诱发Bruch膜破裂后,予10g/L(20mg/kg)柚皮素,1次/d,持续4wk;光凝后2,4wk分别做眼底荧光血管造影,评估CNV的形成。采用彩色微球技术、眼电生理技术检测兔眼血流和大鼠视网膜功能恢复。结果:与对照组比较,10g/L柚皮素能明显增加高眼压兔眼脉络膜血流(P<0.05),能明显增加缺血大鼠眼视网膜功能恢复(P<0.05),能明显减轻光凝点荧光素渗漏(75.8%～95.0%,P<0.01)。结论:柚皮素能抑制激光诱发大鼠络膜新生血管形成;增加高眼压兔眼脉络膜血流;增加缺血大鼠眼视网膜功能恢复。

AIM： To investigate the effects of naringenin on laser- induced experimental choroidal neovascularization （CNV） in rat models, ocular blood flow in rabbit eyes and retinal function recovery after ischemic insults in rat eyes. METHODS： Male Brown Norway rats were treated to break the Bruch＇s membrane. Naringenin 10g/L （20mg/kg） was given once-daily through intraperitoneal injection for 4 weeks after laser treatment. The development of CNV was determined by fluorescein angiography （FA） performed on weeks 2 and 4. The colored microsphere technique and electroretinography method were used for the study of ocular blood flow and retinal function recovery, respectively. RESULTS： The choroidal blood flow in elevated intraocular pressure （lOP） rabbit eyes was significantly increased by 10g/L naringenin solution as compared to control group （ P 〈 0.05） . The retinal function recovery after ischemic insults in rat eyes indicated significant increase of b-wave recovery in treated group, as compared to control group （P 〈 0. 05）. The intensity of fluorescein leakage from the photocoagulated lesions decreased significantly in treated group, compared to the control group（75.8%-95.0%, P〈 0.01 ）. CONCLUSION： Naringenin could prevent the development of CNV on laser-induced experimental rat models, increase the choroidal blood flow in elevated lOP rabbit eyes and be beneficial on retinal function recovery in ischemic rat eyes.